RO   EN PROJECT LEADER UNIVERSITY OF MEDICINE AND PHARMACY CLUJ-NAPOCA
AMPEROMETRIC IMMUNOSENSOR FOR OVARIAN AND UTERINE CANCER BIOMARKERS


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Stages


YEAR 2011
1. Electrode transducer choice, oct. 2011 dec. 2011
1.1. Bioelement choice or preparation
  1.1.1. Bioreceptor choice (antibodies), oct. 2011 dec. 2011
1.2. Imunosensor preliminary testing (immobilization method)
  1.2.1 Choice of the immobilization method for normal and tumoral antiMUC1 onto various electrode materials (conductive polymers); oct. 2011 dec. 2011

YEAR 2012
1. 1. Electrode material choice and design (oct. 2011-mar. 2012)
  1.1. 1.Use of carbon based and gold screen-printed electrodes and magnetic particules
1. 2. Imunosensor design and bioelement choice, oct. 2011 mar. 2012.
  1.2.1. Assay design with antibodies
1.3. Imunosensor development and optimization (various immobilization methods)
  1.3.1. Normal and tumoral antiMUC1 antibodies choice, various immobilization methods, oct. 2011-mar. 2012
  1.3.2. Fabrication and optimization of the graphite and gold SPE modified with antibodies or aptamers, jan. dec. 2012
2. Electroanalytical characterization of the new immunosensors
2.1. Electrochemical behavior study of new immunosensors for MUC1 (in cooperation with University of Florence (Italy) jul. dec. 2012

YEAR 2013
1. Electroanalytical characterization of the new immunosensors
  1.1. Electrochemical behavior study of new immunosensors for MUC1 (in cooperation with University of Florence, Italy and Claude Bernard Universty Lyon, France)
2. Elaboration of the imunoelectrochemical method
2.1. Immunoreaction study of normal and tumoral MUC1 solutions by using CV, DPV, SWV, amperometry or impedance spectroscopy (in cooperation with Oncological Institute Cluj-Napoca
2.2. Experimental design and optimization (influence of the antibody concentration reaction time, temperature, interferences, etc)

YEAR 2014
1. Elaboration of the imunoelectrochemical method
1.1. Ab-Ag reaction study with the second antivody labeled with HRP, alkaline phosphatase, tirosynase and the electroanalytical detection of H2O2, phosphates or polyphenols (in cooperation with University of Florence, Italy and Claude Bernard Universty Lyon, France)
2. Real sample analysis
2.1. Real sample analysis (healthy and cancer pacients) an the comparison with other currently used methods (imunohistochenistry, etc) in cooperation with Oncological Institute Cluj-Napoca

YEAR 2015
1. Achievement and testing of other bioreceptors (molecularly imprinted polymer, MIP type)
1.1. Development of a MIP for a cytostatic applied in ovarian cancer treatment (in cooperation with Claude Bernard University Lyon, France)
1.2. Testing of the new MIP based sensor on synthetic samples

YEAR 2016
1. Testing of the achieved immunosensors on spiked and real samples
1.1. Testing of the immunosensors based on magnetic particles, antibodies and aptamers on real samples
1.2. Comparison of the obtained results with other methods (ELISA, immunohistochemistry)